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OCR: imate the Several different methods are used to e mmonly, free fraction of the total T4 or T3. Most c 251-labelledthe serum of patients is incubated with 1 yroid T3 which occupies binding sites on the t not hormone binding proteins, mainly TBG der is left already occupied by T4, while the remai fractions in solution. The protein-bound and free ich the are separated by addition of a resin, to w ee to free hormone binds. The proportion of 1 to the protein-bound 1251-T3 is inversely relate hyroid residual unoccupied binding capacity of y related to hormone binding proteins, and is direct free hormone fractions. See also: . Thyroid Hormone Transport